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Category: Essay

Subcategory: Biology

Level: PhD

Pages: 2

Words: 1100

Evaluating the possible mechanism of action of a novel anti-fungal drug on cdc20gene expression in AML cell lines
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Acute myeloid leukemia (AML) is fast progressing cancer of the blood which originates in the bone marrow..Increased expression of Cdc20 has been implicated in various cancerous cells and is considered to be a potential target for anti-cancer drugs.
The present study was conducted to evaluate the mechanism of action, of a novel drug used to treat AML. The drug is an established antifungal agent and has shown to cause cytotoxicity in AML cell lines. The evaluation was based on RNA levels and cDNA expressions, derived from AML cell lines compared to non-cancerous cell lines.
Reverse transcription was used to get the cDNA imprints. The qRT-PCR approach was implemented to evaluate the changes in expression of RNA samples (based on cDNA expressions).
The Cycle Threshold (CT) signals were detected as the positive reaction in the qRT-PCR assay. The average CT values were highest for the sample that was treated by drug for the cdc20 gene. This indicated the novel drug must have reduced the overall expression of cdc20 gene compared to the samples that were untreated with the novel drug.
The time bound evaluation the 2^-ddCT values were evaluated. These values indicated the change of expression of cDNA in the same sample with respect to an endogenous control over a period of time. It was indicated that the…

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