Polyacrylamide Gel Electrophoresis
Electrophoresis is the transportation of particles with charges by an electrical field. Any charged particle placed in an electrical field will migrate to opposite charge electrode. The experimental is useful in to determine the rate of migration of most biological polymers in an electric area, but the movement will be proportional to the charge of the ions (Shi & Jackowski, 1). Zonal electrophoresis is essential as an analytical and preparatory method in a study involving proteins and nucleic acids. Being that most common matrix used is polyacrylamide gels then experiment can also be called gel electrophoresis. Gel electrophoresis is vital in determining the molecular mass of proteins using a property of retarding movement of macromolecules.
To determine the molecular mass of yeast alcohol dehydrogenase (ADH) then the knowledge of the sample yeast present in a mixture is essential. The gel electrophoresis process works on a fundamental principle of size other f molecules (Shi & Jackowski, 1). All the yeast under observation must have similar charge density which is ratio comparing charge and mass. Proteins must also undergo treatment using anionic detergent where mostly is sodium dodecyl sulfate that will separate the subunits by denaturing proteins.
Rf = the distance of yeast (cm)/dye front distance (cm)
A dye which is bromophenol blue is mixed the protein sample. The dy…
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