How to generate induced pluripotent stem cells

HOW TO GENERATE INDUCED PLURIPOTENT STEM CELLS
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Introduction
Having the self-renewal potential and the ability to differentiate into different types of cells, stem cells, especially the embryonic stem cells (ESC), have gained a lot of attention over the past few years. Just as ESC studies have played a significant role in understanding the underlying reproduction mechanisms, cell differentiation, and de-differentiation, as well as their development, important efforts have also been made in the biomedical research of the ESC in the past few years. One of the main areas of research has been the production of the iPSC. There are many ways of generating iPSC, but this paper looks at the process of generating induced pluripotent stem cells by reprogramming fibroblasts in humans with the aid of stemgent human TF lentivirus set.
Discussion
The process begins with the reprogramming the human ES/iPS cells. This process takes approximately seven days. Reprogramming is then followed by the preparation of the MEF conditioned medium ADDIN CSL_CITATION { “citationItems” : [ { “id” : “ITEM-1”, “itemData” : { “DOI” : “10.3791/1553”, “ISBN” : “1940-087X (Electronic)”, “ISSN” : “1940-087X”, “PMID” : “19997062”, “abstract” : “In 2006, Yamanaka and colleagues first demonstrated that retrovirus-mediated delivery and expression of Oct4, Sox2, c-Myc and Klf4 is capable of inducing the pluripotent state in mouse fibroblast…