Antibiotic Sensitivity Experiment
Antibiotics are substances which are synthesized by one organism and exhibit toxicity to other organisms. The substance is said to have selective toxicity. This is the basis of differentiating substances which are useful and those which are harmful. Antibiotics which inhibit the growth of pathogenic organisms without causing harmful effects have therapeutic and economic benefits. A proposal by suggests that a selective inhibitor reacts with a substance from a specific sensitive organism. There is also a possibility that antibiotic inhibits a reaction which is atypical to the parasite.
Broad-spectrum antibiotics attack a wide range of bacteria. They are powerful and flexible when dealing with bacterial infections. A good example is Ampicillin which has the ability to destroy Gram-negative and Gram-positive bacteria. On the other hand, narrow-spectrum antibiotics attack specific bacteria. They can be used to prevent the treatment form damaging useful bacteria in the body. These antibiotics are less likely to cause re-infection to a patient with a resistant bacterium.
MATERIALS AND METHODS
This Kirby-Bauer test experiment uses Muller-Hinton medium which is high in protein. The experiment aims at determining the sensitivity of different bacteria species like S. epidermidis to different types of antibiotics.
Materials. The experiment needs two Mueller-Hinton agar plates, 24-hour cultur…
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