DNA Topology and the Initiation of Virus DNA Packaging
Q1. The title of the article is “DNA Topology and the initiation of virus DNA packaging” and the author Oh, Choon Seok and the rest. The report can be found from EBSCOhost which is an online library.
Q2. The topic is relevant since as a science student it makes me understand how large viruses of dsDNA uses hydrolysis from ATP motor to package DNA (Oh et al. 2) into empty shells known as proheads.
Q3. The main topic of the article is on an understanding of how DNA topology initiates the DNA packaging of a virus. The process was determined using various models to show that viral DNA is identified by the subunit of DNA which is smaller (TerS) while the major subunit of terminase comprises of ATPase domains and translocations of endonuclease in lambda phage (Oh et al. 4). The process involves a recognition that takes place through the interactions of viral enzymes which are packaging, terminates and recognition sites of viral DNA.
Q4. Plasmids do not interfere with the helper phage production, showing that 12 re18-50 functions in cis in the background of plasmid and they are cis-specific
Q5. The model will be fundamental in the future to determine the number of other problems associated with the large-scale structure of DNA (Oh et al. 12). The method applies to generate future ensembles of linear particles to compute the distribution of catenated and knotted isomer that occurs afte…
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