Bacterial Morphology and Gram Staining
Gram staining technique is a biological lab method used to classify two distinct groups of bacteria. The differentiation is based on the components of the bacterial cell wall. In this case, the Gram stains differentiate between the Gram-Positive and Gram-negative through red and violet coloring of the cells. The existence of peptidoglycan cell membrane in the bacteria is a significant aspect that facilitates the classification of the bacteria as either the Gram-negative or Gram-positive type of bacteria. In the case of Gram-positive bacteria, they have a dense layer of peptidoglycan cell membrane which is the primary cause of violet stains during this process. Gram-negative bacterium, however, has a skinny layer of peptidoglycan in its cell membrane which is the primary cause of red stains. In this case, the size of the peptidoglycan structure in the cell walls of the bacteria is the main distinguishing factor in the Gram Staining procedure (Bottone, 288-290).
The Gram Staining technique is based on four basic steps which result in the classification of different types of bacteria based on their cell morphology. The primary steps in this technique are as follows;
Application of crystal violet (CV), the primary stain. This is the first step in the Gram Staining process. The aqueous solution of the CV dissociates into Cl- and CV ions. Then, these ions pene…
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